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The anti-dermatophytic activity was evaluated both by agar diffusion method and micro wells dilution method against the The free radical scavenging activity values were ranged from 0.7 to 1.41 mg/ml and 0.6 to 1.23 mg/ml, respectively for leaf and stem bark extracts.
The SCa50 values were calculated by linear regression of plots, where the abscissa represents the concentration of the tested plant extracts and the ordinate the average percent of scavenging capacity.
This method depends on the reduction of purple DPPH to a yellow coloured diphenyl picrylhydrazine.
The determination of the disappearance of free radicals was done using UV-visible spectrometer (Multiskan Spectrum, Thermo Scientific).
Therefore, the present objectives of this study are to evaluate its leaf and barks extracts for anti-dermatophytic properties which is used in folk medicine in Manipur, India as well as to find its chemical profiles such as total phenol, antioxidants and its flavonoids using three organic solvents with different polarity (petroleum ether, chloroform and methanol) for extraction. The plant material was identified by Botanical Survey of India, Kolkata and the herbarium voucher sample (WR234) was deposited in the Departmental Herbarium.500 g of shade-dried pulverized plant materials were subjected to extraction in a Soxhlet apparatus successively with petroleum ether, chloroform and methanol, the volume of each solvent was 6 times the volume of plant extract.
For each solvent, extraction was conducted until no more coloured matter was extracted.